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Attach the transfer head assemblies in the orientation of which you wish to take your sample from. Meaning one will have pressure attached to it to force out the water sample(fig. 12). While the other will have a sample manifold(fig. 13) that will drain into a sample container.
fig. 12 
fig. 13
3. Open the sample chamber
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Keep tool set up just as it was being sampled from with both transfer head assemblies attached. the goal is to rinse anything that would come in contact with the sample water as it is being extracted. Place the tool in a secure vertical position. Fill up a carboy with at least 1.8L of DI water and run a line from the carboy into the lower transfer head assembly. From the upper assembly and sample manifold run a hose into a (chamber*) that is then connected to a vacuum pump. If set up correctly(fig. 1414a, b) once the pump is turned on it will suck the water through the tool effectively rinsing it three times. Remember not to have the openings of the (chanberchamber*) directly opposite each other as the water will spray and some may get into the pump.
fig. 14a 
fig. 14b
2. Wipe and dry out
After the tool is rinsed the transfer head assemblies can be removed and the upper and lower valve assemblies can be removed along with the push rod that connects them. Wipe down the push rod. Be sure to completely dry the sample chamber and tool components before reassembly.
3. Replace O-rings and lubricant where necessary
The Kuster tool should require little maintenance other than disassembly to clean and check the O-rings and reapplication of lubricant to the threads. At a minimum replace the inside sealing O-ring on both the upper and lower valve assembly(Fig. 15).
fig. 15
Replace the O-rings using the yellow and black tool that was designed and 3D printed for this purpose, found in the Kuster accessories box. Use the tool as follows:
A.