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Expedition 393 (South Atlantic Transect 2) is the last in a series of 4 expeditions in the middle of the South Atlantic. Occupying 4 of the 7 total sites, this expedition also provided an opportunity for repeated downhole activities that potentially captured temporal variations. This is on top of the rare site layout that transect the western flank of the Atlantic mid-oceanic ridge. Given this rare chance unique opportunity for a spatio-temporal coverage, a list of downhole activities were conducted during this expedition: the normal wireline logging, APCT-3 runs, CTD profiling and Niskin bottom water bottle sampling for microbiology.

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