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Scanning Electron Microscope Uploader:
User Guide


Manual Information




Author(s):

S. Herrmann

Reviewer(s):

L. LeVay

Manager Approval (Name, Title, Date):

D.J. Houpt

Audience:

Scientists, Laboratory Technicians

Origination date:

06/07/15

Current version:

V374

March 2018

Previous versions:

V1.1

June 2015

Domain:

Description

System:

Scanning Electron Microscopy

Keywords:

SEM, TM3000, image,

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The MegaUploadaTron5001 (MUT, Figure 1) is the software that will upload the SEM pictures and metadata file to the LIMS database. The program needs to be installed on the PC and up and running in order to have a successful upload take place.



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_Ref302118442_Ref302118442Figure 1. MegaUpoadaTron5001 (MUT) symbol.



Figure 2. MegaUploadaTron5001 (MUT) User interface.

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If the user selected a file that is not one of the image types that the SEM produces (JPG, PNG, or TIF) the following error message will appear (Figure 4):


Figure 4. SEM Uploader message if a NON-image file (JPG, PNG, or TIF) is picked.


If the user selected a file that has no metadata .txt file the following error message will appear (Figure 5):



Figure 5. SEM Uploader message if an image file without metadata file is picked.

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  1. The user now needs to pick a Category from the drop-down list. The red asterisk next to the category field indicates that this field is mandatory information. All the following information is not.
  2. The user can pick a general subcategory.
  3. If the expedition has an expedition specific subcategory associated with it this field will auto populate. If there is no expedition specific subcategory, the field will be greyed out.
  4. The user will then be able to select a value from the drop-down list. However, if the desired value is not in the list, it can't be added by the user. The user will need to ask the description technician to add it to the drop-down list.

Here are examples for adding descriptive metadata to the SEM image:

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Naming and Saving Conventions

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Naming convention for SEM files:

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E.g.
349_U1431A_1H_1_10-11cm_Emiliania_huxleyi_1,
349_U1431A_1H_1_10-11cm_Emiliania_huxleyi_2,
349_U1431A_1H_1_10-11cm_Emiliania_huxleyi_3, …


Storage convention for SEM files on the local PC:

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E.g.
Folder SEM
Folder 1431
Folder 1341A
Folder U1341A_1_H_1_1-11cm (all files for this SEM sample)
All those files need to be moved to USERVOL and data1 before the end of the expedition.