Table of Contents

• 1 Overview
  • 1.1 Instruments
• 2 Sample Preparation
• 3 Sample Imaging
• 4 Scanning Electron Microscopy (SEM)
• 5 Stepper Stages

Open

Microscopy Lab location on Core Deck (the bow of the ship is to the right).

Overview

The Microscopy laboratory is used by shipboard paleontologists to identify microfossils for dating the sediments, by sedimentologists to check grain sizes and more and by igneous petrologists/alteration and structure geologists to classify rocks. We also carry a fluorescent microscope for microbiological sample analysis.

Instruments

• Four Zeiss Stereo binocular microscopes, with manual 8:1 zoom and either reflected or transmitted light viewing

• One Zeiss Axioplan microscope equipped for simultaneous transmitted and reflected polarized light viewing, with a large variety of objectives

• Two Zeiss Axioskop A1 microscopes equipped for simultaneous transmitted and reflected polarized light viewing, with a large variety of objectives

• One Zeiss Axiophot microscope equipped for DIC, phase contrast, and fluorescence studies and for cross-polarized light, plane-transmitted light, and darkfield illumination

• On Zeiss Imager 2 fluorescent microscope and one Zeiss Imager 2 with DIC capability

• Most microscopes are equipped with SPOT cameras for digital microphotography,

• The MBIO epifluorescence microscope has a Zeiss camera attached that works with the Zen blue software

• PICAT to photograph full thin section images in plane- and cross-polarized light (Please ask a technician to take those for you, if they were not automatically produced when you received the thin section.)

• Please note that proprietary manuals are available upon request and subject to limited use

Open

Sample Preparation

• Thin Sections

• Fine Fraction Spraying Quick Start Guide

• SybrGold Virus and Bacteria Direct Count Protocol

Sample Imaging

• PICAT Quick Start Guide (technician will perform this)

• PICAT User Guide (technician will perform this)

• Image Capture Quick Start Guide

• Image Capture: Image Scaling

• Zen blue Image Acquisition on MBIO microscope

Scanning Electron Microscopy (SEM)

• SNE-4500M Plus SEM Quick Start Guide

• SEM-EDS Bruker Quick Start Guide

• Sputter Coater Quick Start Guide

• Hitachi SEM User Guide 

• Hitachi SEM Quick Start Guide 

Stepper Stages

• Automatic Stepper Stage/Point Counter