Microscope Image Scaling
Manual Information
Author(s): | D. Houpt (362T) | |
Reviewer(s): |
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Management Approval (Name, Title, Date): | D. Houpt (371T) | |
Audience: | Users of microscopes | |
First Release: | Draft | July 2016 (Exp. 362T) |
Current Version: | 374 | March 2018 |
Revised: | R. Gray, Z. Mateo | |
Domain: | Microscopy | |
System: | Microscope Image Capture |
Contents
Manual Information
Method Overview
JRSO Microscope Environment
Upright Microscopes
What the User Sees
What the SPOT Flex Camera Sees
Optovar
Stereo Microscopes
What the User Sees
What the SPOT Idea Camera Sees
Selected Magnification
Calculating the Scale Bar
Selected (Physical) Objectives
Virtual Objectives
Full CCD/CMOS Acquisition
Procedure for Science User
Start of Expedition
Before Taking a Sample Image
Expected Error
| Table of Contents |
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Method Overview
Slide specimens are examined by a variety of Zeiss microscopes and images are acquired using Diagnostic Instruments SPOT microscope cameras. The images are then annotated by the user in the Image Capture software, then the software calculates the scaling and inserts a scale bar into the image prior to upload into the Laboratory Information Management System (LIMS) database.
The scale bar calculation is based upon the magnification of the microscope optical path (from adapter tube to objective) and the area of the charged-coupled device/complementary metal-oxide-semiconductor (CCD/CMOS) chip in the particular model of SPOT camera. It is not directly based on the field of view (FOV), a fact that has caused some confusion in the past.
All magnification in the optical path—including the Optovar found on several upright microscopes and the magnification wheel on all of the stereo microscopes—must be accounted for or the calculated scale bar will not be accurate.
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