Microscope Image Scaling
Manual Information
Author(s): | D. Houpt (362T) | |
Reviewer(s): |
| |
Management Approval (Name, Title, Date): | D. Houpt (371T) | |
Audience: | Users of microscopes | |
First Release: | Draft | July 2016 (Exp. 362T) |
Current Version: | 374 | March 2018 |
Revised: | R. Gray, Z. Mateo | |
Domain: | Microscopy | |
System: | Microscope Image Capture |
Contents
Manual Information
Method Overview
JRSO Microscope Environment
Upright Microscopes
What the User Sees
What the SPOT Flex Camera Sees
Optovar
Stereo Microscopes
What the User Sees
What the SPOT Idea Camera Sees
Selected Magnification
Calculating the Scale Bar
Selected (Physical) Objectives
Virtual Objectives
Full CCD/CMOS Acquisition
Procedure for Science User
Start of Expedition
Before Taking a Sample Image
Expected Error
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Figure 1. Imaging area options in the SPOT software.
In the example shown in Figure 1, the SPOT camera will only record an image within the yellow square, which represents only 10.4% of the full area of the screen. This means that the area of the subsampled image will be 9.6x as large in two dimensions; this is equivalent to a factor of 3.1x in one dimension. The scale bar recorded in the image will therefore be 3.1x too small as shown in Figure 2 because the Imaging Area parameter is not passed by the SPOT camera to the Image Capture software. The JRSO does not have a solution to this, so remember to always keep the SPOT software in "Full Chip" mode!
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