FROSTING SLIDES: USER GUIDE

INTRODUCTION

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1. Connect vacuum hose to the right forward vacuum plugin (Fig. 3).

Figure 3

1. Check oil level in the vacuum pump under the counter.
2. Turn on vacuum pump with the button labeled Vacuum under the joystick on the LP50.
3. Close valve to the left of the vacuum gauge (Fig. 4).
4. The vacuum is less than 150mbar (Fig. 4). It should be in the green portion of the gauge.

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Vacuum ValveExhaust FlapVacuum Gauge
Figure 4

1. 1. If there is not a proper vacuum seal right away try pressing lightly on the slides to help them seal and make sure the exhaust flap (Fig. 4) is clean.
   2. If that does not work, take off the slides and clean them and chuck face again.
2. Gently set the jig upright on the lapping plate, in the forward arm (Fig. 5).
3. The plate monitor can stay in place on the back arm (Fig. 5).

Figure 5

1. Turn on the abrasive drum by overriding and make sure it is dripping properly.
2. Reset the timer and set it to 18

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   minutes.
3. Set the plate speed to 5 rpms.
4. When the plate is wet press Start.
5. Increase the plate speed in increments to 58 rpms.
6. The jig should start to spin after a couple of minutes when all the slides even out to the same thickness.
   1. Bungee cords can be added to the vacuum hose to assist in helping the jig spin (Fig. 5).
   2. Do not walk out of the lab as the LP 50 is running.
7. When time is up, press Stop.
   1. It is best to bring the speed back down to 5 rpms after the lapping plate is stopped, to help prevent starting it back up at a high speed.
8. Take the jig off of the plate carefully.
9. Open vacuum valve and turn it off.
10. Place jig chuck face up in the sink (Fig. 6).

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1. Hold the micrometer with your pinkie and ring finger through the "C" part of the micrometer. Your index finger and thumb will move the dial (Fig. 7).

Figure 7

1. Clean the micrometer before use by placing a piece of paper in between the two bars and closing it gently (do not apply force). Pull the paper out and repeat.
   1. Gently close the micrometer all the way gently. It should read zero.
2. Place the slide in between the two bars and close them gently onto the slide (Fig. 8).

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1. 1. Take a measurement at the middle and sides to make sure that the frosted slide is even all the way across.
   2. Uneven slides will cause your sample to be uneven. If this happens put the slide back onto the jig and lap it down longer until it is even.
2. Organize the frosted slides into groups based on slide thickness (Fig. 9).

Figure 9